This antibody is suitable for use in Flow Cytometry. This clone has also been reported to be useful for Immunohistochemistry (both Frozen andParaffin Sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
FLOW CYTOMETRY ANALYSIS:
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Neutrophils constitute the principal cells of acute inflammation.Their entrance is stimulated by chemotactic factors secreted from injured cells, resident tissue macrophages, and complement activation. Neutrophils can also be activated by Fc region of antibodies, and by T-cell derived cytokines. A very potent chemotactic factor for neutrophils is C5a, a peptide product of complement activation. Neutrophils contain abundant cytoplasmic granules, which contain toxic proteins. (clinical correlete:type III, roitt) They are short lived cells. They engulf microorganisms, destroy it, but die quickly thereafter. Neutrophils are activated by antibodies, complement and cytokines.