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CD4 抗体 (PE)

CD4 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal CT-CD4 PE
产品编号 ABIN114151
发货至: 中国
  • 抗原 See all CD4 抗体
    CD4
    适用
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    小鼠
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    大鼠
    克隆类型
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    单克隆
    标记
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    This CD4 antibody is conjugated to PE
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    Flow Cytometry (FACS)
    特异性
    This monolconal antibody (mAb) recognizes mouse CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells.
    克隆位点
    CT-CD4
    亚型
    IgG2a
    Top Product
    Discover our top product CD4 Primary Antibody
  • 应用备注
    Flow Cytometry (see Protocols). (Reported to be useful in immunohistochemistry on acetone fixed frozen sections. )
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: PE Rat IgG2a
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS, 0.1 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原
    CD4
    别名
    CD4 (CD4 产品)
    背景
    Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC's and in signal transduction via protein tyrosine kinase lck1.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
    基因ID
    12504
    NCBI登录号
    NP_038516
    UniProt
    P06332
    途径
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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