Suitable for use in Western Blot and ELISA. Recommended Chromogens: Diaminobenzidine or TMB for immunohistochemistry,4-chloro napthol for Immunoblotting and0-phenylenediamine or ABTS for Enzymeimmunoassay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
1-2 mg/mL (OD280)
缓冲液
Saline Phosphate containing 0.02 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized product at 2 - 8 °C for up to one month or at -20 °C for longer. Following reconstitution store in aliquots at -20 °C.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Synonyms: Fluorescein Isothiocyanate