E. Coli 抗体 (FITC)
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- 抗原 See all E. Coli products
- E. Coli
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适用
- E. coli
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This E. Coli antibody is conjugated to FITC
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应用范围
- Immunofluorescence (IF)
- 免疫原
- Mixture of E. coli serotypes
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- 应用备注
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Indirect Immunofluorescence (titer >1: 1,600) assay vs serotypes O157: H7, O20, O125, O55,O111 and K12. Direct Fluorescenceimmunoassay staining of target antigens in a permissive tissue culturesystem. Acetone fixation of the antigen source is recommended prior to staining. Enzymeamplification following reaction with FITC conjugate can also be accomplished utilizingenzyme-antibody conjugates specific to FITC.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 4-5 mg/mL (OD280 nm, E 0.1% = 0 1.4)
- 缓冲液
- 0.01 M PBS, pH 7.2 with 0.1 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
- 背景
- Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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