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Transferrin 抗体 (N-Term)

Name: Transferrin 抗体 (N-Term)
SKU: ABIN112497
Availability: OutOfStock

TF 适用: 人, Pig, 兔 WB, IP, IF, EIA, RIA, Func 宿主: 小鼠 Monoclonal HTF-14 unconjugated

(4 references)

产品编号 ABIN112497

发货至: 中国

Datasheet as PDF

抗原 See all Transferrin (TF) 抗体

Transferrin (TF)
抗原表位
8

7

7

5

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
136

38

37

35

13

13

8

8

7

6

5

4

4

4

3

3
人, Pig, 兔
宿主
154

59

23

14

4
小鼠
Compatible Secondaries
克隆类型
190

60

1
单克隆
标记
136

37

33

12

8

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This Transferrin antibody is un-conjugated
应用范围
159

90

69

49

44

31

30

25

23

19

16

15

13

12

10

9

8

7

6

6

6

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Functional Studies (Func)

特异性

The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

交叉反应（详细）

Species reactivity (expected):Porcine and Rabbit.
Species reactivity (tested):Human.

纯化方法

Precipitation Methods and Ion Exchange Chromatography

免疫原

Purified Porcine Transferrin.

克隆位点

HTF-14

亚型

IgG1

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TF 适用: 人 WB, IF 宿主: 兔 Polyclonal RB55977 unconjugated

应用备注

ELISA. RIA. Western Blotting. Immunocytochemistry. Functional Application: This antibody blocks binding of Transferrin to the Receptor. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Positive Control Tissue: Placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Laube, Heister, Scholz, Borchardt, Braun: "Re-programming of newt cardiomyocytes is induced by tissue regeneration." in: Journal of cell science, Vol. 119, Issue Pt 22, pp. 4719-29, (2007) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, (1985) (PubMed).

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." in: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, (1982) (PubMed).
抗原

Transferrin (TF)

Abstract

TF 产品

背景

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 mmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

基因ID

7018

NCBI登录号

NP_001054

UniProt

P02787

途径

Transition Metal Ion Homeostasis