Keratin 3 抗体
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- 抗原 See all Keratin 3 (KRT3) 抗体
- Keratin 3 (KRT3)
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适用
- 人, 兔, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.
- 交叉反应 (详细)
- Species reactivity (tested):Human, Rabbit and Bovine.
- 纯化方法
- Ion Exchange Chromatography.
- 免疫原
- Human epithelial keratin
- 克隆位点
- AE5
- 亚型
- IgG1
- Top Product
- Discover our top product KRT3 Primary Antibody
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- 应用备注
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Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Keratin 3 (KRT3)
- 别名
- Cytokeratin 3 (KRT3 产品)
- 别名
- CK3 antibody, K3 antibody, CK-3 antibody, keratin 3 antibody, KRT3 antibody
- 背景
- Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3
- 基因ID
- 3850
- NCBI登录号
- NP_476429
- UniProt
- P12035
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