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SYNPO 抗体

Name: SYNPO 抗体
SKU: ABIN112223
Availability: OutOfStock

SYNPO 适用: 人, 小鼠, 大鼠, Cow, Gerbil, 豚鼠 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal G1D4 unconjugated

(5 references)

产品编号 ABIN112223

发货至: 中国

Datasheet as PDF

抗原 See all SYNPO 抗体

SYNPO (Synaptopodin (SYNPO))
适用
29

29

25

2

2

2

1

1

1
人, 小鼠, 大鼠, Cow, Gerbil, 豚鼠
宿主
43

4

3
小鼠
Compatible Secondaries
克隆类型
46

4
单克隆
标记
19

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SYNPO antibody is un-conjugated
应用范围
38

14

11

8

6

6

6

5

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.

交叉反应（详细）

Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.

纯化方法

Affinity chromatography on Protein A

免疫原

Isolated Rat kidney glomeruli.

克隆位点

G1D4

亚型

IgG1

Top Product

Discover our top product SYNPO Primary Antibody
anti-Synaptopodin (SYNPO) (AA 331-452), (Isoform 2) antibody
SYNPO 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 97-300) antibody
SYNPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 331-452), (Isoform 2) antibody
SYNPO 适用: 小鼠, 大鼠 IHC, ICC 宿主: 豚鼠 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 601-700) antibody
SYNPO 适用: 小鼠, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 601-700) antibody (FITC)
SYNPO 适用: 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal FITC

anti-Synaptopodin (SYNPO) (C-Term) antibody
SYNPO 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 601-700) antibody (PE)
SYNPO 适用: 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal PE

anti-Synaptopodin (SYNPO) (AA 1-260) antibody
SYNPO 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Synaptopodin (SYNPO) (AA 601-700) antibody (Biotin)
SYNPO 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal Biotin

anti-Synaptopodin (SYNPO) (AA 601-700) antibody (AbBy Fluor® 555)
SYNPO 适用: 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal AbBy Fluor® 555

应用备注

Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 1 mL distilled water

缓冲液

Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Tan, Rak-Raszewska, Skovorodkin, Vainio: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).

Xu, Chen, Dong, Meyuhas, Chen: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).

Yates, Papakrivopoulou, Long, Goggolidou, Connolly, Woolf, Dean: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).
抗原

SYNPO (Synaptopodin (SYNPO))

别名

Synaptopodin / SYNPO (SYNPO 产品)

背景

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029

基因ID

11346, 9606

UniProt

Q8N3V7