ACTC1 抗体
(3 references)-
- 抗原 See all ACTC1 抗体
- ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))
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适用
- 人, 兔, 小鸡, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ACTC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
- 交叉反应 （详细）
- Species reactivity (tested):Human, Bovine, Rabbit and Chicken.
- 纯化方法
- Affinity Chromatographyon Protein G.
- 免疫原
- Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
- 克隆位点
- AC1-20-4-2
- 亚型
- IgG1
- Top Product
- Discover our top product ACTC1 Primary Antibody
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anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) antibody
ACTC1 适用: 人 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) (AA 156-368) antibodyACTC1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) (N-Term) antibodyACTC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) antibodyACTC1 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) (AA 156-368) antibodyACTC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) antibodyACTC1 适用: 人, 大鼠, 兔, Pig, 山羊 WB, ELISA, IHC, IHC (fro) 宿主: 小鼠 Monoclonal 22D3 unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) (AA 156-368) antibodyACTC1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) antibodyACTC1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, Alpha, Cardiac Muscle 1 (ACTC1) (N-Term) antibodyACTC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in 1.0 mL distilled water.
- 缓冲液
- Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).
: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." in: Circulation, Vol. 120, Issue 25, pp. 2559-66, (2009) (PubMed).
: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).
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: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).
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- 抗原
- ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))
- Abstract
- ACTC1 产品
- 别名
- acta1 antibody, actc antibody, fa27h01 antibody, fb83f06 antibody, wu:fa27h01 antibody, wu:fb83f06 antibody, ACTC antibody, ASD5 antibody, CMD1R antibody, CMH11 antibody, LVNC4 antibody, Actc-1 antibody, actcl antibody, actc1l antibody, cmd1r antibody, ACTC1 antibody, actin, alpha, cardiac muscle 1 L homeolog antibody, actin, alpha, cardiac muscle 1b antibody, actin, alpha, cardiac muscle 1 antibody, actin, alpha, cardiac muscle 1a antibody, actc1.L antibody, actc1b antibody, ACTC1 antibody, Actc1 antibody, actc1a antibody, actc1 antibody
- 背景
- Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
- 基因ID
- 70
- NCBI登录号
- NP_005150
- UniProt
- P68032
- 途径
- Myometrial Relaxation and Contraction
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