ADRP 抗体 (N-Term)
(5 references)-
- 抗原 See all ADRP (PLIN2) 抗体
- ADRP (PLIN2) (Perilipin 2 (PLIN2))
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抗原表位
- AA 5-27, N-Term
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适用
- 人, 大鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ADRP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Polypeptide Reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data), apparent Mr 52,000 (after SDS-PAGE), pI 6.72. Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested Reactivity on Cultured Cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK. Negative with glioma.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Rat and Dog. Negative with Bovine and Mouse.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Synthetic peptide corresponding to aa 5-27 from N-terminus of Human Adipophilin.
- 克隆位点
- AP125
- 亚型
- IgG1
- Top Product
- Discover our top product PLIN2 Primary Antibody
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anti-Perilipin 2 (PLIN2) (AA 1-29), (N-Term) antibody
PLIN2 适用: 人 WB, IHC (p), IHC (fro) 宿主: 豚鼠 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 168-437) antibodyPLIN2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 286-437) antibodyPLIN2 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2C5H8 unconjugated
anti-Perilipin 2 (PLIN2) antibodyPLIN2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 31-130) antibodyPLIN2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 245-433) antibodyPLIN2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 5-232) antibodyPLIN2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 249-376) antibodyPLIN2 适用: 人 WB, ELISA, Coat, FACS 宿主: 小鼠 Monoclonal ADFP-1365 unconjugated
anti-Perilipin 2 (PLIN2) (AA 1-437) antibodyPLIN2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Perilipin 2 (PLIN2) (AA 249-376) antibodyPLIN2 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 兔 Monoclonal ADFP-2755R unconjugated Recombinant Antibody
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- 应用备注
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Immunohistochemistry on Frozen Tissue: 1/10 in PBS, pH 7.4(See Ref.17 for staining protocols). Immunohistochemistry on Paraffin-Embedded Tissue (After microwave treatment). Incubate 1 h at room temperature or over night at 2-8 °C. Cytological Material. Immunoblotting (Western blot): 0.2 μg/mL using ECL has been reported for previous batchnumbers.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL distilled water
- 缓冲液
- Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store the lyophilized antibody at -20 °C.
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: "Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models." in: Veterinary research communications, Vol. 46, Issue 4, pp. 1175-1193, (2022) (PubMed).
: "Lipid droplet dynamics in healthy and pyometra-affected canine endometrium." in: BMC veterinary research, Vol. 18, Issue 1, pp. 221, (2022) (PubMed).
: "Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease." in: BMC medical genomics, Vol. 11, Issue 1, pp. 111, (2019) (PubMed).
: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).
: "Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins." in: Histochemistry and cell biology, Vol. 124, Issue 5, pp. 445-52, (2005) (PubMed).
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: "Lipid droplets and perilipins in canine osteosarcoma. Investigations on tumor tissue, 2D and 3D cell culture models." in: Veterinary research communications, Vol. 46, Issue 4, pp. 1175-1193, (2022) (PubMed).
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- 抗原
- ADRP (PLIN2) (Perilipin 2 (PLIN2))
- 别名
- Adipophilin / ADFP (PLIN2 产品)
- 背景
- Adipophilin / ADRP is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al., for review see e.g. Targett-Adams et al.), inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).Synonyms: ADRP, Adipose differentiation-related protein
- 基因ID
- 123
- NCBI登录号
- NP_001113
- UniProt
- Q99541
- 途径
- Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
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