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- 抗原
- Macrophages
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Recognises an epitope of a membrane protein which is associated with a receptor for glycoconjugates (Sialoadhesin). Antigen Distribution: Isolated Cells: TRPM-3 is negative with Monocytes and Granulocytes. Tissue Sections: TRPM-3 stains marginal zone macrophages and marginal metallophils of the spleen strongly. It rarely reacts with cordal Macrophages in the red pulp and interdigitating cells in the periarterial lymphatic sheet of the spleen. In lymph nodes, only sinus Macrophages express the antigen. Moreover, it is expressed on macrophages in the synovial membrane, mucosa and submucosa of the gastrointestinal tract, twisted spindle-shaped macrophages of the omentum and on macrophages in the dermis and subcutaneous tissue. It is absent from macrophages in the thymus, Peyer's patches and from Langerhans cells in the skin.
- 交叉反应 （详细）
- Species reactivity (tested):Rat (Macrophages).Negative in Human, Mouse, Hamster, Guinea Pig, Goat and Rabbit.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Peritoneal Macrophages.
- 克隆位点
- TRPM-3
- 亚型
- IgG1
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anti-Macrophages (pan) antibody
适用: 大鼠 IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal Ki-M2R unconjugated
anti-Macrophages antibody适用: Pig FACS, IHC (fro), WB, IP 宿主: 小鼠 Monoclonal BA4D5 unconjugated
anti-Macrophages antibody适用: 人, 大鼠, Pig, 犬, Cat, Cow, 兔, Baboon, 山羊, 豚鼠, 马, 猕猴, Cynomolgus FACS, IHC (fro), IHC (p), IHC 宿主: 小鼠 Monoclonal MAC387 unconjugated
anti-Macrophages antibody (FITC)适用: 人, 大鼠, Pig, 犬, Cat, Cow, 兔, Baboon, 山羊, 豚鼠, 马, 猕猴, Cynomolgus FACS 宿主: 小鼠 Monoclonal MAC387 FITC
anti-Macrophages (pan) antibody适用: 小鼠 IHC (fro) 宿主: 大鼠 Monoclonal MOMA1 unconjugated
anti-Macrophages (pan) antibody适用: 小鼠 FACS, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
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- 应用备注
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Suitable for Immunohistochemistry on Frozen Sections: 0.25-0.5 μg/mL (1/400-1/800) when using an ABC amplification system. Suggested Positive Control: Rat Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2, with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Macrophages
- 背景
- TRPM-3 is a specific cell surface marker useful for the identification of tissue fixed rat macrophages and macrophage subpopulations. It is particularly suitable for phenotyping macrophages of lymphoid organs, where it reacts with marginal zone macrophages and with marginal metallophils. It is also an interesting marker for studying autoimmune diseased tissues. TRPM-3 recognises an epitope of a membrane protein which is associated with a receptor for glycoconjugates (sialoadhesin).Synonyms: Macrophage marker
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