Immunohistochemistry on Frozen Sections: 10 μg/mL (1/40)Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Microwave pretreatment forantigen retrieval is recommended. Recommended Positive Control: Rat spleen. Has been described to work in ELISA and Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Reconstitute by adding 0.5 mL distilled water.
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
AIMP1
(Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))
EMAPII is a proinflammatory cytokine and chemoattractant of macrophages. The 20 kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E-selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free calcium concentration, inducing TNFalpha and tissue factor and stimulating chemotaxis. EMAP II has been shown in a tumor model to sensitize the local tumor vasculature to the action of TNFalpha, rendering resistant tumors sensitive to immunotherapy by TNFalpha.Synonyms: AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, EMAP-II, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1