Soluble Terminal Complement Complex (sC5b-9) 抗体
sC5b-9
适用: 人
FACS, IHC (fro), IHC (p)
宿主: 小鼠
Monoclonal
X-11
unconjugated
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- 抗原
- Soluble Terminal Complement Complex (sC5b-9)
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应 （详细）
- Species reactivity (tested):Human: Follicular dendritic (reticulum) cells, C9 neoepitope.No cross reaction observed when tested with polymerized complement C9 of 38 different species, including Mouse, Rat, Rabbit and Guinea Pig.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Enriched Human blood dendritic cells. Remarks: The antigen is a neoepitope of C9 after assembly in the terminal complement complex. Epitope sequence has not been determined.
- 克隆位点
- X-11
- 亚型
- IgG1
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- 应用备注
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Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/200). Microwave pretreatment forantigen retrieval is recommended. Has been described ro work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7min. at 700Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2, 0.1 % Kathon, 5 mg/mL BSA
- 储存液
- Kathon CG
- 注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- 抗原
- Soluble Terminal Complement Complex (sC5b-9)
- 别名
- Complement SC5b-9 (TCC / MAC)
- 背景
- Follicular dendritic cells and sinus lining cells in lymph node and tonsil, and blood dendritic cells express the X-11 antigen. X-11+ blood dendritic cells show typical dendritic veils and are the most potent stimulator cells in an allogenic mixed leukocyte reaction when compared with other leukocytes. X11+ cells strongly react with anti S100 and weakly with anti CD68. The literature suggests that X-11+ cells are dendritic cells of monocytic origin.Synonyms: Marker for Complement C9 Neoepitope On Follicular Dendritic Cells, Membrane attack complex, Terminal complement complex
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