MHC Class II (I-A K,s,r) 抗体
适用: 小鼠
FACS, IHC (fro)
宿主: 大鼠
Monoclonal
ER-TR2
unconjugated
1 image
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- 抗原
- MHC Class II (I-A K,s,r)
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography
- 免疫原
- Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.
- 克隆位点
- ER-TR2
- 亚型
- IgG2b
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- 应用备注
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water (=stock solution).
- 浓度
- 0.2 mg/mL
- 缓冲液
- Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2 - 8 °C.
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- 抗原
- MHC Class II (I-A K,s,r)
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