MHC Class I, H-2 抗体
适用: 小鼠
FACS, IHC (fro)
宿主: 大鼠
Monoclonal
ER-HR52
unconjugated
1 image
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- 抗原
- MHC Class I, H-2
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Mouse macrophage precursor cells Remarks: MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta2-microglobulin (11.5 kDa). The epitope recognized by ER-HR52 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.
- 克隆位点
- ER-HR52
- 亚型
- IgG2a
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1 μg/mL (1/200). Recommended Positive Control: Mouse Spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!
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- 抗原
- MHC Class I, H-2
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