Mucin-Like Carcinoma Antigen (MCA) 抗体
适用: 人
IHC (fro), IHC (p)
宿主: 小鼠
Monoclonal
b-12
unconjugated
1 image
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- 抗原
- Mucin-Like Carcinoma Antigen (MCA)
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Breast carcinoma cell lines.
- 克隆位点
- B-12
- 亚型
- IgG1
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- 应用备注
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Immunohistochemistry on: Frozen sections: 0.5 μg/mL (1/200). Paraffin sections: 4 μg/mL (1/25), Proteinase K pretreatment for antigen retrievalrecommended. Suggested positive control: Human uterus. Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is onlylocated at the MCA producing sites such as glandular cell surfaces or glandular tubuli. InMCA producing tumors, where cells become disorganized, the b-12 antigen is secreted intostromal tissue and blood vessels. (See Table 1. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, buffer pH 7.2 with 0.05 % Sodium Azide as preservative and 2 mg/mL BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Mucin-Like Carcinoma Antigen (MCA)
- 背景
- Synonyms: Marker for Mucin Producing Cells
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