hCG 抗体
This anti-hCG antibody is a 小鼠 单克隆 antibody detecting hCG in EIA 和 IHC (p). Suitable for 人.
Quick Overview for hCG 抗体 (ABIN111952)
抗原
See all hCG 抗体
hCG
(Chorionic Gonadotropin (hCG))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This hCG antibody is un-conjugated
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
H6
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特异性
- This Monoclonal antibody Clone h6 recognizes β-HCG, a well defined oncofetal protein of 36.7 kDa m.w. associated with pregnancy and some cancers. Raised serum levels for HCG are found in teratogenic carcinomas and trophoblastic tumors and occasionally in gastrointestinal, urogenital, bronchial or mammary carcinomas, and in lymphomas.
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纯化方法
- Affinity Chromatography
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亚型
- IgG1
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anti-Chorionic Gonadotropin (hCG) antibody
hCG 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chorionic Gonadotropin (hCG) antibodyhCG 适用: 人 WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.1 μg/mL (1/4000)Paraffin sections: 0.1 μg/mL (1/4000), microwave pretreatment for antigen retrieval isrecommended. It works also in ELISA. Suggested positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- hCG (Chorionic Gonadotropin (hCG))
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物质类
- Hormone
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背景
- Human gonadothropin is a glycoprotein of 36.7 kDa consisting of two subunits. The α-chain (MW 14.5 kD) is common to three other glycoprotein hormones (TSH, FSH, LH) whereas the β-chain (22.2kD) is more different. The β-chain of HCG and LH (lutrophin) have sequence homology in the first 112 amino acids and differ at the C-terminal end consisting of 24 amino acids.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta
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基因ID
- 1082
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NCBI登录号
- NP_000728
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UniProt
- P01233
抗原
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