Endothelial Cells 抗体
适用: 人
IHC (fro)
宿主: 小鼠
Monoclonal
A10-33-1
unconjugated
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- 抗原 See all Endothelial Cells products
- Endothelial Cells
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Endothelial Cells antibody is un-conjugated
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应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity chromatography.
- 免疫原
- Human melanoma cell lines.
- 克隆位点
- A10-33-1
- 亚型
- IgG1
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anti-Endothelial Cells antibody
适用: Pig IHC (fro), EIA 宿主: 小鼠 Monoclonal PE1 unconjugated
anti-Endothelial Cells antibody适用: 人 IHC (fro) 宿主: 大鼠 Monoclonal 1F10 unconjugated
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- 应用备注
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Immunohistochemistry on frozen sections: 0.5-1 μg/mL (1/200). This product does not stainparaffin sections (The antigen is located on the cell surface and sensitive toformaldehyde). Suggested Control: Tissue sections of human liver, lung or skin. Antigen Distribution: A 10-33/1 recognizes endothelial cells in various human, murine andbovine organs (lung vs. liver) and endothelial cell cultures. It is a very useful marker for thecharacterization of primary melanomas, particularly in relation to prognosis of metastasisand when combined with macrophage marker 25F9. In preliminary experiments thisantibody reacted strongly with purified GPIIb-IIIa (CD41/CD61 or aIIbb3) which is expressedon platelets but not on endothelial cells. The antibody also reacted strongly with integralmembrane proteins extracted from platelets, but not with thrombospondin in a directELISA. Immunoprecipitation of the antigen from human lung carcinoma revealed two bandsof apparent molecular weight of 50 and 60 kD, respectively, under reduced conditions, and50 and 120 kD, respectively, under non reducing conditions. CD61 (b3), which is stronglyexpressed on platelets and endothelial cells in combination with CD51 (av, vitronectinreceptor) and shares the b3 subunit with GPIIb-IIIa, has molecular weights of 90 and110 kD, respectively, under non reducing and reducing conditions. Thus, the nature of theantigen remains to be elucidated.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum For further information and details see technicalinformation.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.6 mL distilled water.
- 浓度
- 0.12 mg/mL
- 缓冲液
- PBS, pH 7.2, 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- Endothelial Cells
- Abstract
- Endothelial Cells 产品
- 背景
- The antigen is GPIIb-IIIa located on the cell surface, found on the surface of isolated human, bovine and murine endothelial cells from various organs. It is also found on various tumorous cells such as melanoma, teratoma, euroblastoma and carcinoma cells. The antigen is absent on glioblastoma and on T and B lymphoblastoid cells.Synonyms: Endothelial Cell marker
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