Interdigitating Cells (Access. T-Cell Macrophages) 抗体
适用: 人
FACS, IHC (fro), IHC (p)
宿主: 小鼠
Monoclonal
X-12
unconjugated
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- 抗原
- Interdigitating Cells (Access. T-Cell Macrophages)
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Affinity chromatography
- 免疫原
- Human mononuclear peripheral blood cells.
- 克隆位点
- X-12
- 亚型
- IgG1
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- 应用备注
-
Suitable for Immunohistochemistry on frozen sections (0.25 μg/mL, 1/800) and FACS. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
-
- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS pH 7.2 containing 0.01 % thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
-
- 抗原
- Interdigitating Cells (Access. T-Cell Macrophages)
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