CD33 抗体
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- 抗原 See all CD33 抗体
- CD33
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD33 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography
- 克隆位点
- MY9
- 亚型
- IgG2b
- Top Product
- Discover our top product CD33 Primary Antibody
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- 应用备注
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Immunohistochemistry (IHC) on frozen sections: 4 μg/mL (1: 100). FACS: 10 l stock solution to label 10^6 cells. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2, 0.05 % (v/v) Kathon CG, 5 mg/mL BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2-8 °C after reconstitution.
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- 抗原
- CD33
- 别名
- CD33 / SIGLEC3 (CD33 产品)
- 别名
- SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody
- 背景
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67 kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
- 基因ID
- 945
- NCBI登录号
- NP_001763
- UniProt
- P20138
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