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CD14 抗体 (Biotin)

CD14 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal X-8 Biotin
产品编号 ABIN111908
发货至: 中国
  • 抗原 See all CD14 抗体
    CD14
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    小鼠
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    This CD14 antibody is conjugated to Biotin
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    纯化方法
    Affinity chromatography.
    克隆位点
    X-8
    亚型
    IgG2b
    Top Product
    Discover our top product CD14 Primary Antibody
  • 应用备注
    Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 μg/mL,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15 % positiveHL-60 stimulated with DMSO 15 % positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 0.5 mL distilled water.
    浓度
    0.4 mg/mL
    缓冲液
    PBS, pH 7.2 with 5 mg/mL bovine serum albumin (BSA) as a stabilizer without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody at -20 °C. This product should be stored undiluted.
  • 抗原
    CD14
    别名
    CD14 (CD14 产品)
    背景
    CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
    基因ID
    9606
    UniProt
    P08571
    途径
    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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