CD14 抗体 (Biotin)
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- 抗原 See all CD14 抗体
- CD14
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD14 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity chromatography.
- 克隆位点
- X-8
- 亚型
- IgG2b
- Top Product
- Discover our top product CD14 Primary Antibody
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- 应用备注
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Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 μg/mL,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15 % positiveHL-60 stimulated with DMSO 15 % positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2 with 5 mg/mL bovine serum albumin (BSA) as a stabilizer without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody at -20 °C. This product should be stored undiluted.
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- 抗原
- CD14
- 别名
- CD14 (CD14 产品)
- 背景
- CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
- 基因ID
- 9606
- UniProt
- P08571
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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