S100A8 抗体 (C-Term) (Biotin)
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- 抗原 See all S100A8 抗体
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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抗原表位
- C-Term
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适用
- 人, 大鼠, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This S100A8 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
- 交叉反应 （详细）
- Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).
- 纯化方法
- Affinity Chromatography
- 免疫原
- Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.
- 克隆位点
- S13-67
- 亚型
- IgG1
- Top Product
- Discover our top product S100A8 Primary Antibody
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anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibody
S100A8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibodyS100A8 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, Cat, 犬, 猴, 马, 豚鼠, Baboon, 山羊 IHC, StM 宿主: 兔 Monoclonal MAC3157R unconjugated Recombinant Antibody
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, Cat, 犬, 猴, 马, 豚鼠, Baboon, 山羊 IHC, StM 宿主: 小鼠 Monoclonal rMAC3781 unconjugated Recombinant Antibody
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- 应用备注
- Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.
- 限制
- 仅限研究用
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- 溶解方式
- Restore in 0.5 mL distilled water to make stock solution.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- 抗原
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- 别名
- S100A8 / Calgranulin-A / MRP8 (S100A8 产品)
- 背景
- MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
- 基因ID
- 9606
- UniProt
- P05109
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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