Immunohistochemistry on Frozen Sections: 1 μg/mL (1/100). Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/10). No antigen retrievalrequired, microwave treatment may enhance the reactivity. Suggested Positive Control: Rat spleen. Has been described to work in FACS. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.5 mL distilled water.
浓度
0.1 mg/mL
缓冲液
PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium azide as a preservative
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
B-Cells
背景
B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.Synonyms: B cells, B-cell marker