Granulocytes 抗体
适用: 大鼠
FACS, IHC (p), IHC (fro)
宿主: 小鼠
Monoclonal
RK-4
unconjugated
1 image
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- 抗原
- Granulocytes
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Affinity Chromatography
- 免疫原
- Peritoneal cells.
- 克隆位点
- RK-4
- 亚型
- IgG1
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anti-Granulocytes antibody
适用: Pig FACS, IP, WB 宿主: 小鼠 Monoclonal 6D10 unconjugated
anti-Granulocytes (pan) antibody适用: 大鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal RK-4 unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1.25 μg/mL (1/400). Immunohistochemistry on Paraffin Sections (After enzyme digestion). Suggested Positive Control: Rat spleen, blood smears. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Staining procedure for frozen ice-cold acetone-fixed tissue sections: (The whole procedure is performed at room temperature)
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water to make stock solution.
- 浓度
- 0.5 mg/mL IgG
- 缓冲液
- Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- 抗原
- Granulocytes
- 背景
- Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells
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