CD209b Antigen (CD209B) 抗体 (Biotin)
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- 抗原 See all CD209b Antigen (CD209B) 抗体
- CD209b Antigen (CD209B)
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Antigen, Epitope: The antigen is a Glutaraldehyde (0.05 %) resistant protein expressed in the cytoplasm and on the cell surface. Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. In combination with MOMA-1 (Cat#SM066P), the murine metallophilic macrophage marker, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages. The antigen recognized by ER-TR9 has recently been shown to be the murine analogue of the human DC-SIGN (Dendritic Cell - Specific ICAM-3 Grabbing Non-Integrin), named SIGN-R1. The antibody reacts with a Mouse subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses. Antigen Distribution Isolated Cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression.Antigen Distribution
- 纯化方法
- Affinity Chromatography
- 免疫原
- Thymus cells.
- 克隆位点
- ER-TR9
- 亚型
- IgM
- Top Product
- Discover our top product CD209B Primary Antibody
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anti-CD209b Antigen (CD209B) (AA 51-150) antibody
CD209B 适用: 小鼠 WB, IF (cc), IF (p), IHC (fro), IHC (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD209b Antigen (CD209B) antibody (APC)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 APC
anti-CD209b Antigen (CD209B) antibody (PE)CD209B 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal 22D1 PE
anti-CD209b Antigen (CD209B) antibodyCD209B 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal ER-TR9 unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1.5 μg/mL (1/200). Suggested Positive Control: Mouse spleen). Does not react on routinely processed Paraffin Sections. Has been reported to work in Flow Cytometry (Use 10 μL stock solution to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water (= 0.3 mg/mL Stock Solution).
- 浓度
- 0.3 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as a preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!
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: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).
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: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).
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- 抗原
- CD209b Antigen (CD209B)
- 别名
- CD209b / DC-SIGNR1 (CD209B 产品)
- 背景
- SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7
- 基因ID
- 69165
- NCBI登录号
- NP_001032889
- UniProt
- Q8CJ91
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