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Histone 3 抗体 (C-Term)

Rockland 100-401-e81 H3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN1118467
Supplier Product No.: 100-401-e81
发货至: 中国
  • 抗原 See all Histone 3 (H3) 抗体
    Histone 3 (H3)
    抗原表位
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    C-Term
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Histone 3 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    100-401-e81
    Supplier
    Rockland
    原理
    Histone H3 Antibody
    交叉反应 (详细)
    Anti-Histone H3 is directed against the human histone 3 protein.
    产品特性
    Synonyms: rabbit anti-Histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody
    纯化方法
    The product was prepared from monospecific antiserum by delipidation and defibrination.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

    Immunogen Type: Conjugated Peptide

    Top Product
    Discover our top product H3 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:100

    Application Note: Histone H3 antibody has been tested for use in ELISA, IHC, and western blot. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:2000

    ELISA Dilution: 1:10000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    83 mg/ml
    缓冲液
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store H3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Rivera-Casas, Gonzalez-Romero, Garduño, Cheema, Ausio, Eirin-Lopez: "Molecular and Biochemical Methods Useful for the Epigenetic Characterization of Chromatin-Associated Proteins in Bivalve Molluscs." in: Frontiers in physiology, Vol. 8, pp. 490, (2017) (PubMed).

  • 抗原
    Histone 3 (H3)
    别名
    Histone H3 (H3 产品)
    背景
    Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.
    基因ID
    8350
    NCBI登录号
    NP_003520
    UniProt
    P68431
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