Follicular Dendritic Cells 抗体
适用: 大鼠
IHC (fro)
宿主: 小鼠
Monoclonal
Ki-M9R
unconjugated
1 image
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- 抗原
- Follicular Dendritic Cells
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Follicular Dendritic Cells antibody is un-conjugated
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应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Species reactivity (tested):Rat.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Rat peritoneal macrophages.
- 克隆位点
- Ki-M9R
- 亚型
- IgG1
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anti-Follicular Dendritic Cells antibody
适用: 大鼠 IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal KI-M9R unconjugated
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- 应用备注
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Suitable for Immunohistochemistry (IHC): Frozen sections: 5 μg/mL (1: 200)Paraffin sections: 50 μg/mL (1: 20), Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Rat spleenAntigen Distribution Isolated cells: Ki-M9R is negative with all peripheral blood cells andcells of the bone marrow or peritoneal cavity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water (stock solution).
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS buffer with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- 抗原
- Follicular Dendritic Cells
- 背景
- Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.
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