Macrophages 抗体
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- 抗原
- Macrophages
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects monocytes, subpopulation of macrophages, interdigitating cells. The antigen is localized on the surface of cells. It is stable to 2 % periodate-lysine-paraformaldehyde fixation. The epitope has not been further characterized. Antigen Distribution: Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % of peritoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
- 交叉反应 （详细）
- Species reactivity (tested):Rat (Monocytes, subpopulation of Macrophages, interdigitating cells).
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Rat Peritoneal Macrophages.
- 克隆位点
- RM1
- 亚型
- IgG1
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anti-Macrophages (pan) antibody
适用: 大鼠 IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal Ki-M2R unconjugated
anti-Macrophages antibody适用: Pig FACS, IHC (fro), WB, IP 宿主: 小鼠 Monoclonal BA4D5 unconjugated
anti-Macrophages antibody适用: 人, 大鼠, Pig, 犬, Cat, Cow, 兔, Baboon, 山羊, 豚鼠, 马, 猕猴, Cynomolgus FACS, IHC (fro), IHC (p), IHC 宿主: 小鼠 Monoclonal MAC387 unconjugated
anti-Macrophages antibody (FITC)适用: 人, 大鼠, Pig, 犬, Cat, Cow, 兔, Baboon, 山羊, 豚鼠, 马, 猕猴, Cynomolgus FACS 宿主: 小鼠 Monoclonal MAC387 FITC
anti-Macrophages (pan) antibody适用: 小鼠 IHC (fro) 宿主: 大鼠 Monoclonal MOMA1 unconjugated
anti-Macrophages (pan) antibody适用: 小鼠 FACS, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 2 μg/mL (1/100). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/10). Microwave pretreatment forantigen retrieval is recommended. Has been described to work in FACS. Suggested Positive Control: Rat spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2 - 8 °C.
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- 抗原
- Macrophages
- 背景
- The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.Synonyms: Macrophage marker
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