(1 validation)-
- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
- 请咨询
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宿主
- 小鼠
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
- 序列
- DYKDDDDK
- 免疫原
- A synthetic peptide DYKDDDDK conjugated to KLH.
- 亚型
- IgG1
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyD 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyIP 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- WB (1:1000-10000), IP (1:100-500), IF (1:200-1000). Other applications have not been tested. The optimal dilutions should be determined by end user.
- 限制
- 仅限研究用
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- by
- Reveal Biosciences
- No.
- #028889
- 日期
- 2013.11.07
- 抗原
- Lot Number
- L21/2013G
- Method validated
- Immunofluorescence
- Positive Control
- HEK293 cells transfected with DYKDDDDK Tag
- Negative Control
- Untransfected HEK293 cells
- Notes
- Signal was detected in positive control samples but not in negative control samples.
- Primary Antibody
- Antibody: DYKDDDDK Tag antibody
- Catalog number: ABIN1112984
- Lot number: L21/2013G
- Secondary Antibody
- Antibody: Goat anti-mouse IgG (H+L) IF488 conjugated antibody
- Lot number: S6/2013S
- Full Protocol
- HEK293 cells were grown directly on coverslips and transfected with a DYKDDDDK tagged protein.
- Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature (RT).
- Fixed cells were rinsed three times in PBS for 5 min each at RT.
- Cells were blocked in 1 X PBS / 5% normal goat serum / 0.3% Triton X-100 for 20 min at RT.
- Cells were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron
- Microscopy Sciences, 25885-05) overnight at 4°C.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Cells were incubated with secondary antibody diluted 1:150 in Universal Antibody Dilution Buffer
- (Electron Microscopy Sciences, 25885-05) for 60 min at RT in dark.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with Fluoro-Gel II with DAPI (Electron Microscopy Sciences Cat. No: 17985-50).
- Stained cells were imaged with an EIDAQ 100 fluorescent microscope.
- Experimental Notes
- None
生效 #028889 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: : Immunofluorescence analysis of cultured cells using DYKDDDDK Tag antibody (Catalog number ABIN1112984, Lot number L21/2013G). Flag is present in the positive control (HEK293 cells transfected with DYKDDDDK Tag panel 1, transfected cells are indicated by green fluorescent staining) and absent from the negative control (untransfected HEK293 cells panel 2). No staining was observed in the isotype (panel 3) and secondary only (panel 4) controls. DAPI was used as a nuclear counterstain (indicated by blue fluorescent staining). Scale bar = 25 µm.]()
Figure 1: : Immunofluorescence analysis of cultured cells using DYKDDDDK Tag antibody (Catalog number ABIN1112984, Lot number L21/2013G). Flag is present in the positive control (HEK293 cells transfected with DYKDDDDK Tag panel 1, transfected cells are indicated by green fluorescent staining) and absent from the negative control (untransfected HEK293 cells panel 2). No staining was observed in the isotype (panel 3) and secondary only (panel 4) controls. DAPI was used as a nuclear counterstain (indicated by blue fluorescent staining). Scale bar = 25 µm.
Full Methods -
- 状态
- Liquid
- 缓冲液
- PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
- 储存液
- Sodium azide, Thimerosal (Merthiolate)
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
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- 抗原
- DYKDDDDK Tag
- 别名
- Flag Tag (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- DYKDDDDK-Tag, or DYKDDDDK octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the DYKDDDDK-Tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.
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