Flow Cytometry: 2 g/5x10e5cells/test. Immunohistochemistry on frozen sections or cell smears (1: 25-1: 50 dilution). Studies of peripheral blood B cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL of distilled water.
浓度
0.2 mg/mL (prior to lyophilization)
缓冲液
PBS containing 1 mg/mL BSA as stabilizer, containing no preservatives
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.Synonyms: HSA, Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, X62 heat stable antigen