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CD9 抗体 (PerCP)

CD9 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal VJ1-20 PerCP
产品编号 ABIN1112245
发货至: 中国
  • 抗原 See all CD9 抗体
    CD9
    适用
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    宿主
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    小鼠
    克隆类型
    • 124
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    单克隆
    标记
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    This CD9 antibody is conjugated to PerCP
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF)
    产品特性
    Monoclonal Mouse Anti-Human CD9 PerCP, is recommended for use in flow cytometry for identification of human platelets.
    克隆位点
    VJ1-20
    亚型
    IgG2a
    Top Product
    Discover our top product CD9 Primary Antibody
  • 应用备注
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    说明

    Peridin-cholophyll-protein complex (Prozyme).

    样品收集
    1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD9 PerCP and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0,2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The conjugate is provided in liquid form in buffer containing Antibody Stabilizer.
    储存液
    Sodium azide
    注意事项
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    储存条件
    4 °C
  • 抗原
    CD9
    别名
    CD9 (CD9 产品)
    别名
    BTCC-1 antibody, DRAP-27 antibody, MIC3 antibody, MRP-1 antibody, TSPAN-29 antibody, TSPAN29 antibody, Tspan29 antibody, cd9 antibody, wu:fb61g04 antibody, zgc:73383 antibody, zgc:85743 antibody, CD9 molecule antibody, CD9 antigen antibody, CD9 molecule a antibody, CD9 antibody, Cd9 antibody, cd9a antibody
    背景
    This antibody reacts with a 25 kDa molecular weight. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals cytoplasmically located. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues.
    途径
    Response to Water Deprivation, Cell-Cell Junction Organization
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