CD9 抗体 (FITC)
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- 抗原 See all CD9 抗体
- CD9
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD9 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD9F is recommended for use in flow cytometry for identification of human platelets.
- 克隆位点
- VJ1-20
- 亚型
- IgG2a
- Top Product
- Discover our top product CD9 Primary Antibody
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes)
- 样品收集
- 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD9 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0,2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD9
- 别名
- CD9 (CD9 产品)
- 别名
- BTCC-1 antibody, DRAP-27 antibody, MIC3 antibody, MRP-1 antibody, TSPAN-29 antibody, TSPAN29 antibody, Tspan29 antibody, cd9 antibody, wu:fb61g04 antibody, zgc:73383 antibody, zgc:85743 antibody, CD9 molecule antibody, CD9 antigen antibody, CD9 molecule a antibody, CD9 antibody, Cd9 antibody, cd9a antibody
- 背景
- This antibody reacts with a 25 kDa molecular weight. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals cytoplasmically located. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues.
- 途径
- Response to Water Deprivation, Cell-Cell Junction Organization
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