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CD8 抗体 (APC)

Name: CD8 抗体 (APC)
Brand: antibodies-online
SKU: ABIN1112229
Availability: OutOfStock

CD8 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal MEM-31 APC

Image no. 1 for anti-CD8 (CD8) antibody (APC) (ABIN1112229) (CD8 抗体 (APC))

1 image

(3 references)

产品编号 ABIN1112229

发货至: 中国

Datasheet as PDF

抗原 See all CD8 抗体

CD8
适用
191

66

25

12

12

7

7

6

6

6

5

5

5

3

3

3

2

2

2

1
人
宿主
215

48

36
小鼠
Compatible Secondaries
克隆类型
261

30

2
单克隆
标记
96

43

34

26

12

7

5

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is conjugated to APC
应用范围
261

64

54

52

49

42

30

21

17

14

14

14

8

5

4

3

2

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

产品特性

Monoclonal Mouse Anti-Human CD8 APC, is recommended for use in flow cytometry for identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen.

克隆位点

MEM-31

亚型

IgG2a

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CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE

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CD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 适用: 人 IHC, IP, WB, ELISA 宿主: 兔 Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody
CD8 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE

anti-CD8 (CD8) antibody (Biotin)
CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC

应用备注

It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

说明

Allophycocyanin (Febico, Far East Bio-Tech Co.).

样品收集

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD8 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

限制

仅限研究用
状态

Liquid

缓冲液

The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

储存液

Sodium azide

注意事项

1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

储存条件

4 °C
Lyle, Christofidou-Solomidou, Liu, Elder, Albelda, Cotsarelis: "Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 4, Issue 3, pp. 296-301, (2000) (PubMed).

Nuckols, Shea, Horenstein, Burchette, Prieto: "Quantitation of intraepidermal T-cell subsets in formalin-fixed, paraffin-embedded tissue helps in the diagnosis of mycosis fungoides." in: Journal of cutaneous pathology, Vol. 26, Issue 4, pp. 169-75, (1999) (PubMed).

Yamagata, Tanaka, Kudo: "A quantitative immunohistochemical evaluation of inflammatory cells at the affected and unaffected sites of inflammatory bowel disease." in: Journal of gastroenterology and hepatology, Vol. 13, Issue 8, pp. 801-8, (1998) (PubMed).
抗原

CD8

别名

CD8 (CD8 产品)

别名

CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

背景

30/32 kDa MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.