Selectin E/CD62e 抗体 (APC)
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- 抗原 See all Selectin E/CD62e (SELE) 抗体
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Selectin E/CD62e antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD62E APC is recommended for use in flow cytometry for identification of human tissue for flow cytometric analysis using 20 �l/106 cells.
- 克隆位点
- TEA2-1
- 亚型
- IgG2b
- Top Product
- Discover our top product SELE Primary Antibody
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anti-Selectin E (SELE) (AA 501-612) antibody
SELE 适用: 人, Pig, 小鼠, 大鼠 WB, ELISA, FACS, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 22-230) antibodySELE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (N-Term) antibodySELE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 108-238) antibodySELE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 260-420) antibodySELE 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) antibodySELE 适用: 小鼠 WB, IHC, ELISA, FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal UZ5 unconjugated
anti-Selectin E (SELE) antibodySELE 适用: 人 ELISA 宿主: 小鼠 Monoclonal B-P7 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells. CD62L is used at studies in a wide range of cell interactions in the vasculature together with diagnostic implications. This product is routinely tested on HUVEC cell line.
- 说明
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Allophycocyanin (Febico, Far East Bio-Tech Co.).
- 样品收集
- 1. Transfer 100 µl of sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD62E APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 别名
- CD62E (SELE 产品)
- 背景
- The antibody is specific to the human antigen E-selectin (ELAM-1, 115 kDa) that is expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. This antibody is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes.
- 途径
- Thromboxane A2 Receptor Signaling
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