CD58 抗体 (FITC)
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- 抗原 See all CD58 抗体
- CD58
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD58 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD58 is recommended for use in flow cytometry.
- 克隆位点
- HI58a
- 亚型
- IgG1
- Top Product
- Discover our top product CD58 Primary Antibody
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anti-CD58 (CD58) (Internal Region) antibody
CD58 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-240) antibodyCD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) (AA 1-214) antibodyCD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 31-130) antibodyCD58 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 40-220) antibodyCD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-241) antibodyCD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) antibodyCD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated
anti-CD58 (CD58) antibody (Biotin)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin
anti-CD58 (CD58) antibody (PE)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE
anti-CD58 (CD58) antibody (APC)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD58 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing.3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD58
- 别名
- CD58 (CD58 产品)
- 别名
- LFA-3 antibody, LFA3 antibody, ag3 antibody, CD58 molecule antibody, CD58 antibody
- 背景
- Recognizes the human CD58 cell surface antigen. CD58 is a cell adhesion molecule, which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium, and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.
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