CD52 抗体 (APC)
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- 抗原 See all CD52 抗体
- CD52
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD52 antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD52 APC is recommended for use in flow cytometry.
- 克隆位点
- HI186
- 亚型
- IgG2b
- Top Product
- Discover our top product CD52 Primary Antibody
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anti-CD52 (CD52) antibody
CD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: Human Monoclonal Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal HI186 APC
anti-CD52 (CD52) (AA 1-61) antibodyCD52 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) (AA 20-50) antibodyCD52 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) antibodyCD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: 兔 Chimeric Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 IHC (f) 宿主: 兔 Monoclonal CD52-2276R unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 unconjugated
anti-CD52 (CD52) antibody (FITC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 FITC
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 APC
anti-CD52 (CD52) antibody (PE)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 PE
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Allophycocyanin (Febico, Far East Bio-Tech Co.) Anti-CD52, HI185, was included in the Sixth International Workshops on Human Leucocyte Defferentiation Antigens.
- 样品制备
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- Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD52 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 3. As with any product derived from biological sources, proper handling procedures should be used. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing.
- 储存条件
- 4 °C
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- 抗原
- CD52
- 别名
- CD52 (CD52 产品)
- 别名
- CD52 antibody, AI463198 antibody, B7 antibody, B7-Ag antibody, CAMPATH-1 antibody, CLS1 antibody, MB7 antibody, CDW52 antibody, CE5 antibody, CD52 molecule antibody, CD52 antigen antibody, CD52 antibody, Cd52 antibody
- 背景
- HI186 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
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