ITGA3 抗体 (PE)
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- 抗原 See all ITGA3 抗体
- ITGA3 (Integrin, alpha 3 (ITGA3))
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适用
- 人
- 宿主
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克隆类型
- 单克隆
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标记
- This ITGA3 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- CD49c, a 150kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is expressed by most cell types including T and B lymphocytes
- 克隆位点
- VJ1-6
- 亚型
- IgG1
- Top Product
- Discover our top product ITGA3 Primary Antibody
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anti-Integrin, alpha 3 (ITGA3) (AA 33-236) antibody
ITGA3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 63-248) antibodyITGA3 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 3F6D6 unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 976-1025) antibodyITGA3 适用: 小鼠, Pig WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 168-276) antibodyITGA3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (AA 165-199), (N-Term) antibodyITGA3 适用: 人 WB 宿主: 兔 Polyclonal RB52792 unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) antibodyITGA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (Internal Region) antibodyITGA3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 3 (ITGA3) (Internal Region) antibodyITGA3 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).
- 样品制备
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- Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD49c PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer.8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- ITGA3 (Integrin, alpha 3 (ITGA3))
- 别名
- CD49c (ITGA3 产品)
- 别名
- CD49C antibody, GAP-B3 antibody, GAPB3 antibody, ILNEB antibody, MSK18 antibody, VCA-2 antibody, VL3A antibody, VLA3a antibody, ITGA3 antibody, AA407068 antibody, integrin subunit alpha 3 antibody, integrin subunit alpha 3 S homeolog antibody, integrin alpha 3 antibody, ITGA3 antibody, itga3.S antibody, Itga3 antibody
- 背景
- The antibody recognizes the VLA-a3 chain of the VLA-3 complex and is reactive with epithelial tissue and basal renal distal tubules.
- 途径
- CXCR4-mediated Signaling Events, Integrin Complex
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