CD24 抗体 (APC)
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- 抗原 See all CD24 抗体
- CD24 (CD24 Molecule (CD24))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD24 antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD24 APC, is recommended for use in flow cytometry for identification of B Cell subset, monocytes and dendritic cells.
- 克隆位点
- SN3
- 亚型
- IgG1
- Top Product
- Discover our top product CD24 Primary Antibody
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anti-CD24 Molecule (CD24) antibody (FITC)
CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 FITC
anti-CD24 Molecule (CD24) (AA 15-80) antibodyCD24 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4F4E10 unconjugated
anti-CD24 Molecule (CD24) (AA 60-80) antibodyCD24 适用: 人, 小鼠, 大鼠 IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) (AA 1-80) antibodyCD24 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 FACS, IHC (fro), CyTOF 宿主: 小鼠 Monoclonal SN3 unconjugated
anti-CD24 Molecule (CD24) antibody (PE)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 PE
anti-CD24 Molecule (CD24) antibody (APC)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 APC
anti-CD24 Molecule (CD24) (AA 30-60) antibodyCD24 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 FACS, IHC, IHC (fro), IF 宿主: 小鼠 Monoclonal ML5 unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C4 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Allophycocyanin (Febico, Far East Bio-Tech Co.).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD24 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0, 09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD24 (CD24 Molecule (CD24))
- 别名
- CD24 (CD24 产品)
- 别名
- CD24A antibody, Cd24a antibody, CD24 molecule antibody, CD24 antibody, Cd24 antibody
- 背景
- The antibody reacts with the CD24 antigen a heavily glycosylated molecule that migrates as a broad band of 35-45 kDa on both reducing and non-reducing SDS gel electrophoresis. CD24 is attached to the cell membrane via a glycosyl phosphatidylinositol (GPI) anchor and is expressed at multiple stages of B cell development, beginning with the bone marrow CD34 positive pro-B cell compartment and continuing through mature surface IgM positive/IgD positive B cells. CD24 is also expressed on the vast majority of B- lineage acute lymphoblastic leukaemias, B cell chronic lymphocytic leukaemias and B cell non-Hodgkin's lymphoma. the normal spleen, mantle zone lymphocytes are CD24 strongly positive, but germinal center cells are CD24 negative or CD24 weakly positive
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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