This antibody is specific for the extracellular domain of the PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with 125I-PDGF-BB. This antibody does not recognize PDGF receptor alpha. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.
PDGFRB
适用: 人
WB, IF
宿主: 小鼠
Monoclonal
1G9
unconjugated
应用备注
Flow Cytometry: 25 μg/mL. Immunoprecipitation. Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 25 μg/mL. Recommended Positive Control: Human breast cancer tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.2 containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3.Synonyms: Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, PDGF Receptor beta, PDGF-R-beta