HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.
交叉反应 (详细)
Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.
C5
适用: 人
WB, IA
宿主: 小鼠
Monoclonal
568
unconjugated
应用备注
ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL distilled water.
注意事项
This product is photosensitive and should be protected from light
储存条件
4 °C
储存方法
Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5