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Vimentin 抗体 (pSer55)

Name: Vimentin 抗体 (pSer55)
SKU: ABIN1109485
Availability: OutOfStock

VIM 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal 4A4 unconjugated

Western Blotting (WB) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin 抗体 (pSer55))

Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin 抗体 (pSer55))

2 images

产品编号 ABIN1109485

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

16

16

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer55
适用
304

119

116

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
158

129

11

6

6

5

2
小鼠
Compatible Secondaries
克隆类型
172

144
单克隆
标记
197

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
262

115

104

96

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

序列

SLYSS-phosphoS55-PGGAYC-KLH

特异性

This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.

交叉反应（详细）

Species reactivity (tested):Human, mouse, rat

纯化方法

Protein A agarose

免疫原

Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.

克隆位点

4A4

亚型

IgG2b

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应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

储存液

Azide free

注意事项

Avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store (in aliquots) at -20 °C.
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

背景

Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM

基因ID

7431

NCBI登录号

NP_003371

UniProt

P08670

途径

Caspase Cascade in Apoptosis