Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
特异性
The antibody clone AF10 is specific to the 240 kDa Erythroid alpha-Spectrin. This antibody is suitable for Various immunochemical reactions to detect Erythroid Spectrins, Identification of Erythroid leukemias (Ref.1).
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Purified
免疫原
Ghost proteins of Human red blood cells. Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.Synonyms: Beta-I spectrin, SPTB1