Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Purified
免疫原
Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.Synonyms: RPP20, Ribonuclease P protein subunit p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7