Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
This Monoclonal antobofdy P193-10 reacts with an internal epitope (amino acids 506-510, VALGK) of the minor vault protein (p193 or VPARP), which is overexpressed in various Human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.
交叉反应 (详细)
Species reactivity (tested):Human.
免疫原
E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
PARP-1 is a nuclear protein that is specifically cleaved by caspase-3 and caspase-6, but not by caspase-1, into a signature apoptotic fragment. PARP-2 and PARP-3 interact with PARP-1. PARP-4, also designated vault poly(ADP-ribose) polymerase (VPARP) and ADP-ribotransferase-like 1 (ADPRTL1), associates with the major vault protein (MVP) and telomerase-associated protein 1 (TEP1) to form vaults, barrel-shaped cytoplasmic ribonucleoprotein particles. PARP-4 localizes mainly to the cytoplasm but is also found in the nucleus. The PARP-4 protein is expressed widely, with highest levels observed in the kidney, and is also detected in skeletal muscle, heart, leukocytes, placenta, lung, liver, spleen, and pancreas. PARP-4 contains a PARP (ADPRT)-like catalytic domain, a C-terminal MVP-interacting domain, a domain with two sequences similar to inter-alpha-trypsin inhibitor, and an N-terminal BRCA1 C-terminus (BRCT) domain, which may be involved in protein-protein interactions.Synonyms: 193 kDa vault protein, ADPRTL1, KIAA0177, PARP-4, PARP-related/IalphaI-related H5/proline-rich, PARPL, Poly [ADP-ribose] polymerase 4, VPARP, Vault poly(ADP-ribose) polymerase