E. Coli 抗体
适用: E. coli
WB, IF, EIA
宿主: 小鼠
Monoclonal
2D7-1
unconjugated
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- 抗原 See all E. Coli products
- E. Coli
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适用
- E. coli
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This E. Coli antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Escherichia coli J5 cells.
- 克隆位点
- 2D7-1
- 亚型
- IgG2b
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- 应用备注
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Immunofluorescence. ELISA: (a 1 mg/mL solution has been used at dilutions of up to 1/1500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS, pH 7.2, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
- 背景
- Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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