Actin 抗体 (C-Term, pan)
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- 抗原 See all Actin 抗体
- Actin
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抗原表位
- C-Term, pan
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适用
- 人, 大鼠, 小鼠, 小鸡, Snake
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Goat anti-Mouse IgG Affinity Chromatography
- 免疫原
- Synthetic Actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
- 克隆位点
- AC-40
- 亚型
- IgG2a
- Top Product
- Discover our top product Actin Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.2 % Sodium Acetate or neutral PBS.
- 浓度
- 0.1 mg/mL
- 缓冲液
- 1.2 % Sodium Acetate containing 2 mg BSA and 0.01 mg Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at -20 °C. After reconstituion store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Actin
- Abstract
- Actin 产品
- 背景
- Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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