Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Rabbit Serum.
纯化方法
Affinity purified
免疫原
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制
仅限研究用
状态
Liquid
溶解方式
deionized water
浓度
Lot specific
缓冲液
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate
储存液
Gentamicin sulfate
注意事项
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
avoid freeze thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.