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Myc Tag 抗体 (AA 410-419) (Biotin)

Name: Myc Tag 抗体 (AA 410-419) (Biotin)
SKU: ABIN1103150
Availability: OutOfStock

WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin

Image no. 1 for anti-Myc Tag (AA 410-419) antibody (Biotin) (ABIN1103150) (Myc Tag 抗体 (AA 410-419) (Biotin))

1 image

产品编号 ABIN1103150

发货至: 中国

Datasheet as PDF

抗原 See all Myc Tag products

Myc Tag
抗原表位
21

21

12

8

6

3

2

1
AA 410-419

适用

请咨询
宿主
75

49

6

5

5

5

1

1
兔
Compatible Secondaries
克隆类型
77

58

10
多克隆
标记
67

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is conjugated to Biotin
应用范围
110

53

42

36

25

23

20

18

15

11

11

6

6

5

4

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

特异性

This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.

纯化方法

Affinity purified

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein

亚型

IgG
anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB 宿主: 山羊 Polyclonal unconjugated

anti-Myc Tag (AA 408-439) antibody
适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody

应用备注

Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

限制

仅限研究用
状态

Liquid

溶解方式

deionized water

浓度

Lot specific

缓冲液

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

avoid freeze thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
抗原

Myc Tag

Abstract

Myc Tag 产品

物质类

Tag