His Tag 抗体 (Biotin)
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- 抗原 See all His Tag 抗体
- His Tag
- 适用
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This His Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 序列
- HHHHHH
- 特异性
- This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
- 纯化方法
- Protein A purified
- 免疫原
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This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Type of Immunogen: Hapten - 克隆位点
- 33D10-D2
- 亚型
- IgG
- Top Product
- Discover our top product His Tag Primary Antibody
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- 应用备注
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Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- avoid freeze thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- 抗原
- His Tag
- Abstract
- His Tag 产品
- 物质类
- Tag
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