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HA-Tag 抗体 (C-Term)

Name: HA-Tag 抗体 (C-Term)
SKU: ABIN1101941
Availability: OutOfStock

WB, ELISA, IP, IHC, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated

Image no. 2 for anti-HA-Tag (C-Term) antibody (ABIN1101941) (HA-Tag 抗体 (C-Term))

Image no. 1 for anti-HA-Tag (C-Term) antibody (ABIN1101941) (HA-Tag 抗体 (C-Term))

2 images

产品编号 ABIN1101941

发货至: 中国

Datasheet as PDF

抗原 See all HA-Tag products

HA-Tag
抗原表位
16

8

5

2

2

1

1
C-Term

适用

请咨询
宿主
77

73

13

8

1
兔
Compatible Secondaries
克隆类型
88

81
多克隆
标记
67

15

13

11

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HA-Tag antibody is un-conjugated
应用范围
130

57

37

25

18

16

14

13

9

6

6

6

4

4

3

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

序列

YPYDVPDYA

特异性

This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

纯化方法

Affinity purified

过滤

Sterile filtered

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

Type of Immunogen: Purified protein

亚型

IgG
anti-HA-Tag antibody
WB, ELISA, IP, IF, IHC 宿主: 小鼠 Monoclonal HA-C5 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 18B11H6 unconjugated

anti-HA-Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody
WB, IF, ChIP 宿主: 兔 Polyclonal RB0411 unconjugated

anti-HA-Tag antibody
WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5E11D8 HRP

anti-HA-Tag antibody
适用: 标记 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-HA-Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated

anti-HA-Tag antibody
ELISA, Coat 宿主: 小鼠 Monoclonal 16-43 unconjugated

anti-HA-Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 12CA5 unconjugated

应用备注

Approved: ChrIP, ELISA, IHC, IHC-P (1:500 - 1:2000), IP, WB (1:2000 - 1:10000)

Usage: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

avoid freeze thaw cycles. Store undiluted.

储存条件

4 °C,-20 °C

储存方法

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
抗原

HA-Tag

别名

Hemagglutinin / HA Tag (HA-Tag 产品)

物质类

Tag

背景

Name/Gene ID: HA Tag

Synonyms: HA Tag