Immunoblotting: use at 1-2 μg/mL. A band of ~90 kDa is detected. Immunohistochemistry: use at 1-10 μg/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium which is identical to that used in the assay system.
浓度
Lot specific
缓冲液
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
This antibody is stable for at least one (1) year at -20°C.
H(+)/Cl(-) exchange transporter 3,[Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity). {UniProtKB:P51790, PubMed:10915634}., [Isoform 2]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C (PubMed:8155321). It could help neuronal cells to establish short-term memory (PubMed:8155321). {PubMed:10915634, PubMed:8155321}.,Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Clcn3 mediates the exchange of chloride ions against protons and functions as an antiporter, contributing to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.,[Isoform 1]: Lysosome membrane, Late endosome membrane, Cell membrane, Early endosome membrane,Chloride channel protein 3, ClC-3, Chloride transporter ClC-3